Function of 5'-nucleotidase in the uptake of adenosine from AMP by human lymphocytes.
نویسندگان
چکیده
The function of 5'-nucleotidase in nucleoside uptake from AMP was investigated in human lymphocytes by comparing the transport in cells containing this enzyme (5'N+) with that in cells deficient in the activity (5'N-). The rate of adenosine and Pi uptake from AMP was 3.9-fold greater in the 5'N+ then in the 5'N- lymphocytes. There was no difference in transport between these cells when incubated with adenosine or Pi. These results indicate that phosphorylytic cleavage of AMP by 5'-nucleotidase is necessary for the uptake of the nucleotide and Pi moieties by the human lymphocyte.
منابع مشابه
Function of 5’-Nucleotidase in the Uptake of Adenosine from AMP by Human Lymphocytes*
The function of 5’-nucleotidase in nucleoside uptake from AMP was investigated in human lymphocytes by comparing the transport in cells containing this enzyme (5’N+) with that in cells deficient in the activity (5’N-). The rate of adenosine and P, uptake from AMP was 3.9-fold greater in the 5’N+ then in the 5’N lymphoc$tes. There was no difference in transport between these cells when incubated...
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عنوان ژورنال:
- The Journal of biological chemistry
دوره 250 23 شماره
صفحات -
تاریخ انتشار 1975